《植物生理学报》 2010, 46(8): 817-823

拟南芥RopGEFs 家族基因在外源脱落酸处理下的表达分析

王会, 金宇騑, 卢曼曼, 葛光君, 向芬, 李杜, 朱咏华*, 郑巧林*

湖南大学生物学院生物技术系, 长沙410082

通信作者：朱咏华；E-mail: zyh20@hotmail.com, qiaolzheng@hotmail.com；Tel: 0731-88821565, 0731-88664028

摘　要：

ABA 不同浓度和不同时间处理拟南芥野生型7 天龄幼苗, 采用实时荧光定量PCR 方法, 分析小 G 蛋白 ROP10 和ROP 鸟苷酸交换因子 RopGEFs 基因的表达水平差异。结果表明, RopGEF7~10, 12 及13 没有检测到表达, 而RopGEF1~6, 11, 14 及 ROP10 在 ABA 处理下表现出不同的应答趋势, 其中 RopGEF5 基因的表达变化与 ROP10 的变化相似。推测 RopGEF5 有 可能在 ROP10 介导的 ABA 信号转导途经中起调控作用。

关键词：拟南芥; 脱落酸; ROP10; RopGEFs

收稿：2010-04-07   修定：2010-07-16

资助：湖南省科技计划项目(2009FJ3198)。

Expression Analysis of RopGEFs Genes in Arabidopsis thaliana Treated with ABA

WANG Hui, JIN Yu-Fei, LU Man-Man, GE Guang-Jun, XIANG Fen, LI Du, ZHU Yong-Hua*, ZHENG Qiao-Lin*

Department of Biotechnology, College of Biology, Hunan University, Changsha 410082, China

Corresponding author: ZHU Yong-Hua; E-mail: zyh20@hotmail.com, qiaolzheng@hotmail.com; Tel: 0731-88821565, 0731-88664028

Abstract:

The real-time fluorescence quantitative PCR was carried out to analyze the expression profiles of ROP10 (Rho-related GTPase from plants) and RopGEFs (guanine nucleotide exchange factor) genes in 7-d seedlings of Arabidopsis thaliana in response to ABA with different concentrations or periods. The results showed that the expression of RopGEF7-10, 12 and 13 could not be detected in wild type (Col-4) seedlings. RopGEF1-6, 11, 14 and ROP10 indicated different expression patterns in response to ABA treatment, while RopGEF5 showed similar expression profile to ROP10. These results suggested that RopGEF5 might act as a regulate factor in ROP10-dependent ABA signaling pathway.

Key words: Arabidopsis thaliana; ABA; ROP10; RopGEFs